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Journal / Cancer Research / オルガノイド培養を用いたマウス前立腺上皮細胞の分化能力の評価…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
オルガノイド培養を用いたマウス前立腺上皮細胞の分化能力の評価
Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
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Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma
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オルガノイド培養を用いたマウス前立腺上皮細胞の分化能力の評価

Article DOI: 10.3791/60223-v • 10:38 min • November 22nd, 2019
November 22nd, 2019 •
*1, *1, 2, 2, 2,3,4,5,6
1Molecular Biology Interdepartmental Program, University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, 3Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, 4Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, 5Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 6Molecular Biology Institute, University of California, Los Angeles
* These authors contributed equally

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マウス前立腺オルガノイドは、分化を調節するメカニズムを評価する有望な文脈を表す。本論文では、前立腺オルガノイドを確立するための改善されたアプローチについて述べ、(1)オルガノイドからタンパク質リサートを採取する方法、および(2)全マウント共焦点顕微鏡のためのオルガノイドを修正および染色する方法を紹介する。

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がん研究 問題 153 オルガノイド 前立腺 上皮 前駆体 基礎 発光 分化 マウス
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