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Ex Vivo Organoid Model of Adenovirus-Cre Mediated Gene Deletions in Mouse Urothelial Cells
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Chapters
- 00:04Introduction
- 00:45Dissection of Mouse Bladder
- 02:09Dissociation of Urothelial Cells
- 04:41Counting and Resuspending Cells
- 05:27Adenoviral Transduction and Plating Organoids
- 07:06Results: Characterization of Triple Knockout Organoids via Ex Vivo Adenovirus-Cre Recombinase
- 08:31Conclusion
This protocol describes the process of the generation and characterization of mouse urothelial organoids harboring deletions in genes of interest. The methods include harvesting mouse urothelial cells, ex vivo transduction with adenovirus driving Cre expression with a CMV promoter, and in vitro as well as in vivo characterization.
