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Cancer Research

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Culture of Bladder Cancer Organoids as Precision Medicine Tools

Culture of Bladder Cancer Organoids as Precision Medicine Tools

Article DOI: 10.3791/63192-v 08:39 min December 28th, 2021
December 28th, 2021



Patient-derived organoids (PDOs) are a powerful tool in translational cancer research, reflecting both the genetic and phenotypic heterogeneity of the disease and response to personalized anti-cancer therapies. Here, a consolidated protocol to generate human primary bladder cancer PDOs in preparation for the evaluation of phenotypic analyses and drug responses is detailed.


Bladder Cancer Organoids Precision Medicine Urological Cancers Genetic Heterogeneity Phenotypic Heterogeneity Patient Biopsy Downstream Analysis Cellular Basis Molecular Basis Treatments Individual Patients Cancer Tumor Specimen Transport Medium Basal Media Petri Dish Dissection Grid
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