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Culture of Bladder Cancer Organoids as Precision Medicine Tools
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JoVE Journal Cancer Research
Culture of Bladder Cancer Organoids as Precision Medicine Tools

Culture of Bladder Cancer Organoids as Precision Medicine Tools

DOI:
10.3791/63192-v

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08:39 min

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December 28, 2021

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, , , , , , , , , , , , ,
1School of Biomedical Sciences, Faculty of Health,Queensland University of Technology (QUT) at Translational Research Institute, 2Queensland Bladder Cancer Initiative (QBCI), 3Centre for Personalised Analysis of Cancers (CPAC), 4Australian Prostate Cancer Research Centre – Queensland, 5Department of Urology,Princess Alexandra Hospital

Chapters

  • 00:04Introduction
  • 00:48Generation of Bladder Tumor Organoids
  • 06:57Results: Culturing of Patient-Derived Organoids for Translational Cancer Research
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

Patient-derived organoids (PDOs) are a powerful tool in translational cancer research, reflecting both the genetic and phenotypic heterogeneity of the disease and response to personalized anti-cancer therapies. Here, a consolidated protocol to generate human primary bladder cancer PDOs in preparation for the evaluation of phenotypic analyses and drug responses is detailed.

Tags

Keywords: Bladder CancerOrganoidsPrecision MedicineCancer ModelsTumor SpecimenHistopathological AnalysisMolecular AnalysisCryopreservation

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