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使用路西法黄电磷学可视化星形形态
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis
Please note that all translations are automatically generated.
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使用路西法黄电磷学可视化星形形态
DOI:
10.3791/60225-v
•
07:38 min
•
September 14, 2019
•
Stefanie L. Moye
,
Blanca Diaz-Castro
,
Mohitkumar R. Gangwani
,
Baljit S. Khakh
2
1
Department of Physiology, David Geffen School of Medicine
,
University of California Los Angeles
,
2
Department of Neurobiology, David Geffen School of Medicine
,
University of California Los Angeles
Chapters
00:04
Title
00:31
Electrode Preparation
01:25
DYE Ejection Test
02:44
Astrocyte Filling
04:24
Imaging of Filled Astrocyte
05:15
Results: Visualization of Astrocyte Morphology
06:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
星形细胞是形态复杂的细胞,其多重过程和茂密的区域就是例证。为了分析它们的精细形态,我们提出了一种可靠的方案,用于在轻度固定组织中执行细胞内路西法黄色淋病。
Tags
Astrocyte Morphology
Lucifer Yellow Iontophoresis
Astrocyte-neuronal Interactions
Synapse
Brain Slice
Confocal Microscopy
Dye Ejection
Astrocyte Identification
Stratum Radiatum
Hippocampus
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