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Journal / Neuroscience / Imaging van intracellulaire ATP in Organotypic…
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Neuroscience

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JoVE Journal Neuroscience
Imaging van intracellulaire ATP in Organotypic weefsel segmenten van de muis hersenen met behulp van de FRET-gebaseerde sensor ATeam 1.03Yemk
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Imaging van intracellulaire ATP in Organotypic weefsel segmenten van de muis hersenen met behulp van de FRET-gebaseerde sensor ATeam 1.03Yemk

Article DOI: 10.3791/60294-v • 11:20 min • December 19th, 2019
December 19th, 2019 •
*1, *1, 1, 2, 1, 1
1Institute of Neurobiology, Heinrich Heine University Düsseldorf, 2Department of Neurology, Düsseldorf University Hospital
* These authors contributed equally

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We beschrijven een protocol voor cel-type specifieke uitdrukking van de genetisch gecodeerde FRET-gebaseerde sensor ATeam 1.03Yemk in organotypische segment culturen van de muis forebrain. Bovendien, we laten zien hoe deze sensor gebruiken voor dynamische beeldvorming van cellulaire ATP niveaus in neuronen en astrocyten.

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Neurowetenschappen uitgave 154 neurowetenschappen organotypic brain slice astrocyte neuron AAV Hippocampus cortex FRET
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