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Paired Whole Cell Recordings in Organotypic Hippocampal Slices
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Neuroscience
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JoVE Journal Neuroscience
Paired Whole Cell Recordings in Organotypic Hippocampal Slices

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

DOI:
10.3791/51958-v

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09:23 min

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September 28, 2014

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, , ,
1Department of Physiology and Centre for Brain Research,University of Auckland, 2Department of Molecular and Cellular Physiology,Stanford University

Chapters

  • 00:05Title
  • 01:16Making Hippocampal Slices
  • 03:50Handling Hippocampal Slices
  • 05:11Whole Cell Recordings in Hippocampal Slices
  • 07:39Synaptic Connectivity Between CA3 Pyramidal Neurons
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

Pair recordings are simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling precise electrophysiological and pharmacological characterization of the synapses between individual neurons. Here we describe the detailed methodology and requirements for establishing this technique in organotypic hippocampal slice cultures in any laboratory equipped for electrophysiology.

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Keywords: Paired Whole Cell RecordingsOrganotypic Hippocampal SlicesSynaptic ConnectionsElectrophysiologyPatch ClampCA3-CA3 Pyramidal Cell PairsCA3-CA1 PairsSynaptic TransmissionSynaptic Plasticity

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