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Neuroscience

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Paired Whole Cell Recordings in Organotypic Hippocampal Slices
 

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

Article doi: 10.3791/51958
September 28th, 2014

Summary September 28th, 2014

Pair recordings are simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling precise electrophysiological and pharmacological characterization of the synapses between individual neurons. Here we describe the detailed methodology and requirements for establishing this technique in organotypic hippocampal slice cultures in any laboratory equipped for electrophysiology.

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