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Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy
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Neuroscience
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JoVE Journal Neuroscience
Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy

Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy

DOI:
10.3791/56237-v

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08:38 min

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October 23, 2017

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,
1Division of Anatomy, Institute of Basic Medical Sciences,University of Oslo, 2Department of Microbiology,Oslo University Hospital

Chapters

  • 00:05Title
  • 00:46Viral Transduction
  • 01:52Microscope Set-up
  • 03:24Time-lapse Imaging
  • 06:52Results: Time-lapse Imaging to Track Mitochondrial Movement in Oligodendrocytes
  • 08:12Conclusion

Summary

Automatic Translation

Automatic Translation

Myelinating oligodendrocytes promote rapid action potential propagation and neuronal survival. Described here is a protocol for oligodendrocyte-specific expression of fluorescent proteins in organotypic brain slices with subsequent time-lapse imaging. Further, a simple procedure for visualizing unstained myelin is presented.

Tags

Keywords: OligodendrocyteOrganelleOrganotypic Brain SliceAdeno-associated VirusConfocal MicroscopyMitochondriaMyelinTime-lapse ImagingViral Transduction

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