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Mærkning og billeddannelse af amyloid plaques i hjernevæv ved hjælp af den naturlige polyphenol curcumin
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1Field Neurosciences Institute,Ascension St. Mary’s Hospital, 2Field Neurosciences Institute Laboratory for Restorative Neurology,Central Michigan University, 3Program in Neuroscience,Central Michigan University, 4Department of Psychology,Central Michigan University, 5Department of Health Sciences,Saginaw Valley State University
Chapters
- 00:05Title
- 01:04Perfusion
- 02:24Tissue Processing
- 03:48Histochemical A-Beta Labeling of Cryostat Tissue Sections
- 05:00Histochemical Labeling of Paraffin-Embedded Tissue Sections
- 05:42A-Beta-Specific Antibody Labeling
- 07:24Intracellular Amyloid-Beta Protein Colocalization
- 08:00Results: Representative Curcumin-Amyloid Plaque Labeling and Imaging
- 09:24Conclusion
Curcumin er en ideel fluoroforet til mærkning og billeddannelse af amyloid beta protein plaques i hjernevæv på grund af sin præference binding til amyloid beta protein samt dens strukturelle ligheder med andre traditionelle amyloid binding farvestoffer. Det kan bruges til at mærke og billede amyloid beta protein plaques mere effektivt og billigt end traditionelle metoder.
