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Journal
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Medicine
/
マイクロウェル培養を用いた精巣特異的建築を用いたブタ精巣オルガノイドの生成
JoVE Journal
Medicine
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JoVE Journal
Medicine
Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture
Please note that all translations are automatically generated.
Click here for the English version.
マイクロウェル培養を用いた精巣特異的建築を用いたブタ精巣オルガノイドの生成
DOI:
10.3791/60387-v
•
11:53 min
•
October 03, 2019
•
Sadman Sakib
,
Yang Yu
4
,
Anna Voigt
,
Mark Ungrin
3,4
,
Ina Dobrinski
2
1
Biochemistry and Molecular Biology
,
University of Calgary
,
2
Comparative Biology and Experimental Medicine
,
University of Calgary
,
3
Biomedical Engineering Graduate Program
,
University of Calgary
,
4
Alberta Diabetes Institute
,
University of Alberta
Chapters
00:04
Title
00:51
Testis Tissue Enzymatic Digestion
05:22
Germ Cell Enrichment
06:52
Preparation of Cells and Microwells
08:21
Generation of Testicular Organoids
09:51
Results: Testis-specific Tissue Architecture
10:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは、市販のマイクロウェル培養システムを用いた精巣特異的組織アーキテクチャを用いたブタ精巣オルガノイドの再現可能な生成のためのプロトコルを提示する。
Tags
Porcine
Testis
Testicular Organoids
Testis-specific Architecture
Microwell Culture
Testis Morphogenesis
Drug Screening
Toxicity Screening
Enzymatic Digestion
Collagenase
DMEM
Cell Isolation
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