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利用流细胞测定人类白细胞测定阿斯珀吉鲁斯紫锥体烟曲的噬菌体
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Immunology and Infection
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JoVE Journal Immunology and Infection
Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
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利用流细胞测定人类白细胞测定阿斯珀吉鲁斯紫锥体烟曲的噬菌体

DOI:
10.3791/60397-v

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09:43 min

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December 07, 2019

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, , , , , , ,
1Infections in Hematology and Oncology,Leibniz Institute for Infection Biology and Natural Product Research, 2Department for Hematology and Medical Oncology,Jena University Hospital, 3Institute for Transfusion Medicine,Jena University Hospital

Chapters

  • 00:04Title
  • 00:53Preparation of Aspergillus fumigatus Conidia
  • 02:26Preparation of Human Primary Leukocytes
  • 03:22Phagocytosis Assay
  • 04:01Antibody Staining
  • 05:58Flow Cytometry
  • 07:24Results: Measuring Phagocytosis
  • 08:58Conclusion

Summary

Automatic Translation

Automatic Translation

该协议提供了一种快速可靠的方法，用流动细胞测定人类原发性噬菌体定量测量阿苯甲基的邻苯二甲苯，并区分锥体从单纯的粘附到白细胞的邻形图。

Tags

PhagocytosisAspergillus FumigatusConidiaHuman LeukocytesFlow CytometryCell MarkersNeutrophilsMonocytesMucoralesRed Blood CellsFITC LabelingRPMI MediumBuffy CoatEL BufferCentrifugation

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