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Måle fagocytose av Aspergillus fumigatus Conidia av menneskelige leukocytter bruker Flow flowcytometri
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Immunology and Infection
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JoVE Journal Immunology and Infection
Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

Måle fagocytose av Aspergillus fumigatus Conidia av menneskelige leukocytter bruker Flow flowcytometri

English

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6,550 Views

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09:43 min

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December 07, 2019

DOI:

10.3791/60397-v

DOI:
10.3791/60397-v

•

09:43 min
December 07, 2019

•

1 Views
, , , , , , ,
1Infections in Hematology and Oncology,Leibniz Institute for Infection Biology and Natural Product Research, 2Department for Hematology and Medical Oncology,Jena University Hospital, 3Institute for Transfusion Medicine,Jena University Hospital

Summary

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Denne protokollen gir en rask og pålitelig metode for å kvantitativt måle fagocytose av Aspergillus fumigatus conidia av menneskelig primære phagocytes bruker flyt flowcytometri og å diskriminere fagocytose av conidia fra bare vedheft til leukocytter.

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Hartung, S., Rauh, C., Böttcher, S., Hoang, M. T. N., Jahreis, S., Rummler, S., Hochhaus, A., von Lilienfeld-Toal, M. Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry. J. Vis. Exp. (154), e60397, doi:10.3791/60397 (2019).

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