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Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
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Immunology and Infection
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JoVE Journal Immunology and Infection
Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate

Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate

DOI:
10.3791/60466-v

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09:06 min

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February 22, 2020

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, ,
1Department of Biomedical Sciences, Joan C. Edwards School of Medicine,Marshall University, 2Progenesis Technologies LLC,Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine,Marshall University

Chapters

  • 00:04Introduction
  • 00:49SCV Growth Conditions and Physiological Activation of the Salvage Pathway
  • 01:47Uronic Acid Carbazole Assay
  • 04:36ELISA for Alginate Quantitation
  • 06:34Results: Uronic Acid Carbazole Assay, Anti-Alginate mAb-Based ELISA, and Direct Patient Sample Testing
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a growth condition to culture the small colony variant of Pseudomonas aeruginosa. We also describe two separate methods for the detection and quantitation of the exopolysaccharide alginate produced by P. aeruginosa using a traditional uronic acid carbazole assay and an alginate-specific monoclonal antibody (mAb) based ELISA.

Tags

Keywords: Pseudomonas AeruginosaSmall Colony VariantSCVAlginateELISAUronic Acid CarbazolePhysiological ActivationSalvage PathwayOD600

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