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Immunology and Infection

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JoVE Journal Immunology and Infection
Kultur af små koloni variant af Pseudomonas aeruginosa og kvantificering af sin Alginat
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Kultur af små koloni variant af Pseudomonas aeruginosa og kvantificering af sin Alginat

Article DOI: 10.3791/60466-v • 09:06 min • February 22nd, 2020
February 22nd, 2020 •
*1, *2, 1,2,3
1Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, 2Progenesis Technologies LLC, Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine, Marshall University
* These authors contributed equally

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Her beskriver vi en vækst betingelse for at kultur den lille koloni variant af Pseudomonas aeruginosa. Vi beskriver også to separate metoder til påvisning og kvantificering af exopolysaccharid alginat produceret af P. aeruginosa ved hjælp af en traditionel uroonsyre carbazole assay og en alginat-specifik monoklonal antistof (mAb) baseret ELISA.

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Immunologi og infektion Pseudomonas aeruginosa cystisk fibrose (CF) lille kolonivariant (SCV) pyrimidinbiosyntese vækst mucoidy alginat uronic syre carbazole assay alginat-specifik mAb ELISA
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