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Journal / Immunology and Infection / Co-kultur af Murine tyndtarmsepitelorganoider med …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Co-kultur af Murine tyndtarmsepitelorganoider med medfødte lymfoide celler
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Co-kultur af Murine tyndtarmsepitelorganoider med medfødte lymfoide celler

Article DOI: 10.3791/63554-v • 08:22 min • March 23rd, 2022
March 23rd, 2022 •
*1,2, *1,2,3, 1, 1
1Centre for Host-Microbiome Interactions, King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme, King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge University
* These authors contributed equally

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Denne protokol indeholder detaljerede instruktioner til etablering af murine tyndtarmsorganoider, isolering af medfødte lymfoidceller af type 1 fra murine tyndtarmen lamina propria og etablering af 3-dimensionelle (3D) co-kulturer mellem begge celletyper for at studere tovejsinteraktioner mellem tarmepitelceller og type 1 medfødte lymfoide celler.

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Immunologi og infektion udgave 181
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