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1Division of Immunology and Allergy,Lausanne University Hospital, 2Department of Fundamental Neuroscience,University of Lausanne, 3Department of Biochemistry,Erasmus Medical Center, 4Theoretical Biology and Biophysics Group,Los Alamos National Laboratories, 5Department of Biochemistry,University of Lausanne
Chapters
- 00:04Title
- 01:14Reverse Transcription and RNA Isolation
- 02:49Affinity Purification of ASP Biotinylated cDNA
- 04:29Standard PCR
- 05:10Results: ASP RNA Detection in CD4+ Cells from HIV Patients
- 06:56Conclusion
RNA Hårnåle og sløjfer kan fungere som primere for reverse transkription (RT) i fravær af sekvens-specifikke primere, forstyrrer studiet af overlappende antisense udskrifter. Vi har udviklet en teknik, der kan identificere streng-specifik RNA, og vi har brugt det til at studere HIV-1 antisense protein ASP.
