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Chapters
- 00:05Introduction
- 01:13Extraction and Purification of Sporozoites of Eimeria spp. (e.g., E. tenella)
- 03:17Collection and Purification of Merozoites of E. necatrix
- 04:39Nucleofection of Merozoites or Sporozoites
- 05:57Cloacal Inoculation or Intravenous Injection
- 06:36Results: Meronts, Merozoites, Sporocysts, Sporozoites and Oocysts
- 07:20Conclusion
Her har vi givet en metode til at opnå stabil omladning af kylling Eimeria parasitter ved nucleofecting sporozoitter eller anden generation merozoitter. Genetisk modificerede eimeriske parasitter, der udtrykker heterologe antigengene, kan anvendes som vaccinefremføringskøretøjer.
