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Journal
/
Biochemistry
/
热通菌膜-
双磷酶抑制剂的筛选
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Screening for
Thermotoga maritima
Membrane-Bound Pyrophosphatase Inhibitors
Please note that all translations are automatically generated.
Click here for the English version.
热通菌膜-
双磷酶抑制剂的筛选
DOI:
10.3791/60619-v
•
09:11 min
•
November 23, 2019
•
Keni Vidilaseris*
1
,
Niklas G. Johansson*
2
,
Ainoleena Turku
,
Alexandros Kiriazis
,
Gustav Boije af Gennäs
,
Jari Yli-Kauhaluoma
,
Henri Xhaard
,
Adrian Goldman
3
1
Research Program in Molecular and Integrative Biosciences
,
University of Helsinki
,
2
Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy
,
University of Helsinki
,
3
School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology
,
University of Leeds
Chapters
00:04
Title
00:41
Protein Preparation
02:49
Compound Preparation
03:44
Reagents for the Assay Preparation
05:15
Activity Assay for One 96 Well Plate
07:34
Results: Color Development, Curve of the TmPPase Percent Activity, and Inhibition Curve
08:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了膜结合焦磷酶(来自
热托加水红素)
抑制剂的筛选方法,其依据是96孔板格式的二恶极蓝色反应。
Tags
Membrane-bound Pyrophosphatase
Inhibitors
Parasitic Diseases
Reactivation Buffer
Reaction Mixture
Liposomes
Enzyme Reactivation
D-M-S-O
Compound Screening
96-well Plate
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