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Journal
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Immunology and Infection
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微生物通过微血管内皮细胞对血脑屏障渗透性分析
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
微生物通过微血管内皮细胞对血脑屏障渗透性分析
DOI:
10.3791/60692-v
•
06:26 min
•
February 14, 2020
•
Veronika Weber
,
Kaya Bork
,
Rüdiger Horstkorte
,
Heidi Olzscha
1
Institute of Physiological Chemistry
,
Martin Luther University Halle-Wittenberg
Chapters
00:05
Introduction
00:39
Growth of Blood-Brain Barrier Mimicking Cells
02:58
Preparation of Bacteria and Cell Treatment
03:40
Measurement of Permeability
04:46
Results: Effects of Protein Glycation on the Blood-Brain Barrier
05:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
人类血脑屏障选择性地防止亲水分子和病原体渗透到大脑中。几种疾病,包括脑膜炎和术后精神错乱,与血脑屏障的渗透性增加有关。在这里,我们描述了一个内皮细胞培养模型,以测试通过微生物遍历的屏障渗透性。
Tags
Blood-brain Barrier
Microbial Traversal
Microvascular Endothelial Cells
Bacterial Meningitis
Postoperative Delirium
High Throughput Analysis
Collagen IV
Fibronectin
Human Microvascular Endothelial Cells
Trypsin EDTA
FCS
Trypan Blue
E.coli
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