Journal
/
Immunology and Infection
/
Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

DOI:
10.3791/58621-v

•

08:07 min

•

January 07, 2019

•

, ,
1Division of Gastroenterology and Hepatology, Department of Medicine,University of Colorado Anschutz Medical Campus, School of Medicine, 2Department of Immunology and Microbiology,University of Colorado Anschutz Medical Campus

Chapters

  • 00:04Title
  • 00:52Liver Single-cell Suspension Preparation
  • 03:36Flow Cytometric Analysis
  • 05:49Results: Representative Murine Liver Lymphatic Endothelial Cell (LEC) Characterization
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to identify lymphatic endothelial cell populations within the liver using described markers. We utilize collagenase IV and DNase and a gentle mincing of tissue, combined with flow cytometry, to identify a distinct population of lymphatic endothelial cells.

Tags

Keywords: LiverLymphatic Endothelial CellsFlow CytometrySingle-cell SuspensionLiver Biology

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58621?article_type=v

Flow Cytometry Analysis of Immune Cells Within Murine Aortas
check_url/58621?article_type=v

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
check_url/58621?article_type=v

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
check_url/58621?article_type=v

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
check_url/58621?article_type=v

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
check_url/58621?article_type=v

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
check_url/58621?article_type=v

Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10
check_url/58621?article_type=v

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood
check_url/58621?article_type=v

Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells
check_url/58621?article_type=v

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Read Article