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JoVE Journal
Immunology and Infection

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Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells
 

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

Article doi: 10.3791/58621
January 7th, 2019 Usage Statistics

Summary January 7th, 2019

The goal of this protocol is to identify lymphatic endothelial cell populations within the liver using described markers. We utilize collagenase IV and DNase and a gentle mincing of tissue, combined with flow cytometry, to identify a distinct population of lymphatic endothelial cells.

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