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3D多色DNAFISH工具研究人类原细胞核结构
JoVE Journal
Genetics
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JoVE Journal Genetics
3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
DOI:

11:25 min

January 25, 2020

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Chapters

  • 00:04Title
  • 00:45DNA Probe Preparation and Nick Translation Labelling
  • 02:15Human Primary T Cell Fixation, Pre-Treatment, and Permeabilization
  • 04:50Human Primary Myoblast Cell Fixation, Pre-Treatment, and Permeabilization
  • 05:433D multicolor DNA FISH
  • 07:053D Multicolor DNA FISH Detection and Analysis
  • 08:52Results: Representative DNA FISH Analysis of Human Primary Cell Nuclear Architecture
  • 10:45Conclusion

Summary

Automatic Translation

3D 多色 DNA FISH 是一种工具,用于可视化 3D 保留核内的多个基因组位点,明确定义其在单个细胞级别的核空间内的相互交互和定位。在这里,描述了一个分步协议,用于广泛的人类原细胞。

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