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Chapters
- 00:04Title
- 00:45DNA Probe Preparation and Nick Translation Labelling
- 02:15Human Primary T Cell Fixation, Pre-Treatment, and Permeabilization
- 04:50Human Primary Myoblast Cell Fixation, Pre-Treatment, and Permeabilization
- 05:433D multicolor DNA FISH
- 07:053D Multicolor DNA FISH Detection and Analysis
- 08:52Results: Representative DNA FISH Analysis of Human Primary Cell Nuclear Architecture
- 10:45Conclusion
3D multicolor DNA FISH repræsenterer et værktøj til at visualisere flere genomiske loci inden for 3D bevaret kerner, utvetydigt definere deres gensidige interaktioner og lokalisering i det nukleare rum på et enkelt celleniveau. Her beskrives en trinvis protokol for et bredt spektrum af humane primære celler.
