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Journal
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Immunology and Infection
/
肿瘤微环境下免疫细胞种群的可视化、定量和映射
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
Please note that all translations are automatically generated.
Click here for the English version.
肿瘤微环境下免疫细胞种群的可视化、定量和映射
DOI:
10.3791/60740-v
•
11:00 min
•
March 25, 2020
•
Manuel Flores Molina
2
,
Thomas Fabre
2
,
Aurélie Cleret-Buhot
,
Geneviève Soucy
3
,
Liliane Meunier
4
,
Mohamed N. Abdelnabi
2
,
Nicolas Belforte
5
,
Simon Turcotte
4,6
,
Naglaa H. Shoukry
4,7
1
Centre de Recherche du Centre hospitalier de l’Université de Montréal (CRCHUM)
,
2
Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine
,
Université de Montréal
,
3
Département de Pathologie et Biologie Cellulaire, Faculté de Médecine
,
Université de Montréal
,
4
Institut du Cancer de Montréal
,
5
Département de Neurosciences, Faculté de Médecine
,
Université de Montréal
,
6
Département de Chirurgie, Faculté de Médecine
,
Université de Montréal
,
7
Département de Médecine, Faculté de médecine
,
Université de Montréal
Chapters
00:05
Introduction
01:03
Heat-Induced Antigen Retrieval
01:46
Nonspecific Binding Blocking
02:28
Immunofluorescence Labeling
03:36
Image Alignment
04:36
Tissue Detection
05:35
Tissue Segmentation
06:28
Cell of Interest (COI) Identification and Quantification
07:15
Tissue Heatmapping
08:10
Results: Representative Visualization, Quantification, and Mapping of Cell Populations of Interest in the Tumor Microenvironment
10:02
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一种简单易懂的策略,用于在正式固定石蜡嵌入肿瘤组织部分中可视化、量化和映射免疫细胞。该方法将现有的成像和数字分析技术与扩展成像检测的多路复用能力和多参数分析相结合。
Tags
Immune Cell Populations
Tumor Microenvironment
Immunotherapy
Antigen Retrieval
Antibody Staining
Image Analysis
Anti-tumor Response
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