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使用流细胞测定测量红细胞补充受体1
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry
DOI:

07:20 min

May 19, 2020

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Chapters

  • 00:04Introduction
  • 01:15Erythrocyte Washing and Dilution
  • 02:16Erythrocyte Immunostaining and Fixation
  • 03:46Flow Cytometry Analysis
  • 05:47Results: Representative Flow Cytometric anti-CR1 Immunostaining Analyses
  • 06:27Conclusion

Summary

Automatic Translation

该方法的目的是通过与已知红细胞CR1密度的三个受试者进行比较,确定任何受试者红细胞CR1密度的CR1密度。该方法使用抗CR1单克隆抗体在受试者红细胞免疫染色后使用流细胞学,并结合使用植物素(PE)的放大系统。

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