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Journal / Neuroscience / Isolation og udvidelse af neurosfærer fra…
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Neuroscience

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JoVE Journal Neuroscience
Isolation og udvidelse af neurosfærer fra postnatal (P1-3) Mus neurogene nicher
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Isolation og udvidelse af neurosfærer fra postnatal (P1-3) Mus neurogene nicher

Article DOI: 10.3791/60822-v • 10:05 min • May 23rd, 2020
May 23rd, 2020 •
1,2,3, 1,2, 1,2, 1,2, 1,2, 1,2, 1,3, 1,2
1Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 2Instituto de Farmacologia e Neurociências, Faculdade de Medicina, Universidade de Lisboa, 3Instituto de Biologia Molecular, Faculdade de Medicina, Universidade de Lisboa

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I denne artikel beskriver vi i detaljer en protokol for generation af neurosfærekulturer fra postnatale museneurale stamceller fra de primære neurogene nicher. Neurosfærer bruges til at identificere neurale stamceller fra hjernevæv tillader estimering af prækursorcelletal. Desuden kan disse 3D-strukturer være belagt under forskellige forhold, hvilket giver anledning til neuroner, oligodendrocytter og astrocytter, så studiet af celleskæbne.

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Neurovidenskab mus hjerne voksne neurogene nicher neurale stamceller neurosfærer passage spredning differentiering neurale analyser immuncytokemi
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