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Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands
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Immunology and Infection
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JoVE Journal Immunology and Infection
Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands

Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands

DOI:
10.3791/60903-v

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07:26 min

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March 04, 2020

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,
1Institute for Biomedical Sciences,Georgia State University

Chapters

  • 00:05Introduction
  • 00:31Metal Limited Neisseria gonorrhoeae Growth
  • 01:50Metal Loading
  • 02:36Metal-Limited Growth Assay
  • 03:50Outer Membrane Metal Transporter Ligand Binding Detection
  • 05:38Results: Representative Differential Metal Responsive Protein Production, Growth-Restriction, and Binding
  • 06:50Conclusion

Summary

Automatic Translation

Automatic Translation

We describe here a method for growth of Neisseria gonorrhoeae in metal-restricted liquid medium to facilitate the expression of genes for metal uptake. We also outline downstream experiments to characterize the phenotype of gonococci grown in these conditions. These methods may be adapted to be suitable for characterization of metal-responsive genes in other bacteria.

Tags

Keywords: Metal-limited GrowthNeisseria GonorrhoeaeMetal-responsive GenesMetal AcquisitionHost LigandsNutritional ImmunityMetal Restricted ConditionsBacterial GrowthCDMKlett ColorimeterMetal Loaded ProteinsTransferrinDialysis96 Well MicroplateOptical Density

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