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塞里亚淋病的金属有限生长,用于金属反应基因特征和从宿主配体中获取金属
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands
DOI:

07:26 min

March 04, 2020

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Chapters

  • 00:05Introduction
  • 00:31Metal Limited Neisseria gonorrhoeae Growth
  • 01:50Metal Loading
  • 02:36Metal-Limited Growth Assay
  • 03:50Outer Membrane Metal Transporter Ligand Binding Detection
  • 05:38Results: Representative Differential Metal Responsive Protein Production, Growth-Restriction, and Binding
  • 06:50Conclusion

Summary

Automatic Translation

我们在这里描述了一种在金属受限液体介质中生长淋病的方法,以促进金属吸走基因的表达。我们还概述了下游实验,以表征在这些条件下生长的淋球菌的表型。这些方法可以适应其他细菌中金属反应基因的表征。

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