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Immunology and Infection
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在小鼠中进行结肠镜引导捏活检并评估后续组织变化
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes
Please note that all translations are automatically generated.
Click here for the English version.
在小鼠中进行结肠镜引导捏活检并评估后续组织变化
DOI:
10.3791/60949-v
•
08:52 min
•
February 05, 2021
•
David C. Montrose
2
,
Erin M. McNally
,
Erika Sue
,
Andrew J. Dannenberg
1
Department of Pathology, Renaissance School of Medicine
,
Stony Brook University, Stony Brook, NY
,
2
Stony Brook Cancer Center, Stony Brook, NY
,
3
Department of Medicine
,
Weill Cornell Medicine, New York, NY
Chapters
00:05
Introduction
00:46
Colonoscopy and Wound Induction
02:46
Wound Bed Visualization and Measurement
04:37
Wound Bed Collection
06:02
Histological Analysis Preparation
07:45
Results: Representative Wound Bed Visualization
08:21
Conclusion
Summary
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在这里,我们提供了一个详细的程序,诱导小鼠结肠镜引导捏活检和跟踪伤口关闭的实时程序。此外,还提供了组织准备方法,用于对伤口床进行组织学、免疫组织化学和分子分析。
Tags
Colonoscopic-guided Pinch Biopsy
Mice
Colonic Wound Healing
Inflammatory Bowel Disease (IBD) Pathogenesis
Endoscope
Biopsy Forceps
Anesthesia
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