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使用微流体腔系统在运动神经元培养物中有机细胞的斧头迁移
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Neuroscience
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JoVE Journal
Neuroscience
Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System
Please note that all translations are automatically generated.
Click here for the English version.
使用微流体腔系统在运动神经元培养物中有机细胞的斧头迁移
DOI:
10.3791/60993-v
•
10:12 min
•
May 05, 2020
•
Topaz Altman*
1
,
Roy Maimon*
1
,
Ariel Ionescu*
1
,
Tal Gradus Pery
,
Eran Perlson
2
1
Department of Physiology and Pharmacology, Sackler Faculty of Medicine
,
Tel Aviv University
,
2
Sagol School of Neuroscience
,
Tel Aviv University
Chapters
00:04
Introduction
01:08
Microfluidic Chamber Preparation
05:53
Neuronal Culture Plating
07:24
Axonal Transport
08:19
Results: Axonal Transport of Mitochondria and Acidic Compartments in Motor Neurons
09:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
斧头迁移是运动神经元健康的重要机制。在该协议中,我们提供了一种使用微流体室跟踪运动神经元斧头酸性隔间和线粒体的斧头传输的详细方法。
Tags
Axonal Transport
Motor Neuron
Microfluidic Chambers
Neurodegenerative Disease
ALS
Axonal Growth
Axonal Degeneration
PDMS
Chlorotrimethylsilane
Vacuum Desiccator
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