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The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy
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JoVE Journal Neuroscience
The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

DOI:
10.3791/52220-v

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12:18 min

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December 26, 2014

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, , , , , ,
1Physiology and Bosch Institute,University of Sydney, 2Motor Neuron Disease Research Group, Australian School of Advanced Medicine,Macquarie University, 3Advanced Microscopy Facility, Bosch Institute,University of Sydney

Chapters

  • 00:05Title
  • 01:48Unbiased Sampling and En Face Imaging of Motor Endplates
  • 03:41Measuring the Area of Synaptic Specializations in en face Images
  • 07:36Relative Staining Intensities Compared Using Transverse Optical Sections
  • 09:47Results: Measurement of Synaptic Area at the Neuromuscular Junction
  • 11:29Conclusion

Summary

Automatic Translation

Automatic Translation

The neuromuscular junction (NMJ) is altered in a variety of conditions that can sometimes culminate in synaptic failure. This report describes fluorescence microscope-based methods to quantify such structural changes.

Tags

Keywords: Neuromuscular JunctionSynapseConfocal MicroscopyAcetylcholine ReceptorSynaptic VesicleSynaptic ProteinFRETNeurotransmissionMuscle ContractionStructural ChangesMouse Model

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