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Journal
/
Biology
/
C. Elegans의
생리적 스트레스 반응 측정
JoVE Journal
Biology
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JoVE Journal
Biology
Measurements of Physiological Stress Responses in
C. Elegans
Please note that all translations are automatically generated.
Click here for the English version.
C. Elegans의
생리적 스트레스 반응 측정
DOI:
10.3791/61001-v
•
10:36 min
•
May 21, 2020
•
Raz Bar-Ziv*
1
,
Ashley E. Frakes*
1
,
Ryo Higuchi-Sanabria*
1
,
Theodore Bolas
,
Phillip A. Frankino
,
Holly K. Gildea
,
Melissa G. Metcalf
,
Andrew Dillin
1
Department of Molecular and Cell Biology
,
University of California, Berkeley
Chapters
00:04
Introduction
01:00
Stress Response Induction:
hsp-4p::GFP
Readout
02:11
Stereo Microscope or Low-Magnification Wide-Field/Compound Microscope Imaging
04:33
Quantitative Reporter Measurement
06:54
Stress and Heat Sensitivity Measurement
07:54
Results: Representative Cellular Stress Response Evaluation
09:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
여기서, 우리는 선충
C. 예쁜꼬마선충에서
세포 단백질 독성 스트레스 반응을 형광 전사 기의 활성화를 측정하고 생리적 스트레스에 대한 감수성을 측정하여 특성화한다.
Tags
C. Elegans
Physiological Stress Responses
Stress Response Assay
Heat Shock Protein
Endoplasmic Reticulum Unfolded Protein Response
GFP Reporter
Sodium Azide
Micromanipulation
High-throughput
Genetic Perturbations
Pharmacological Perturbations
Molecular And Physiological Analysis
Synchronization
Imaging
Microscopy
Article
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