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Journal
/
Biology
/
C. Elegans
生理应激反应的测量
JoVE Journal
Biology
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JoVE Journal
Biology
Measurements of Physiological Stress Responses in
C. Elegans
Please note that all translations are automatically generated.
Click here for the English version.
C. Elegans
生理应激反应的测量
DOI:
10.3791/61001-v
•
10:36 min
•
May 21, 2020
•
Raz Bar-Ziv*
1
,
Ashley E. Frakes*
1
,
Ryo Higuchi-Sanabria*
1
,
Theodore Bolas
,
Phillip A. Frankino
,
Holly K. Gildea
,
Melissa G. Metcalf
,
Andrew Dillin
1
Department of Molecular and Cell Biology
,
University of California, Berkeley
Chapters
00:04
Introduction
01:00
Stress Response Induction:
hsp-4p::GFP
Readout
02:11
Stereo Microscope or Low-Magnification Wide-Field/Compound Microscope Imaging
04:33
Quantitative Reporter Measurement
06:54
Stress and Heat Sensitivity Measurement
07:54
Results: Representative Cellular Stress Response Evaluation
09:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们通过测量荧光转录机的激活和对生理应激的敏感性来描述线虫
C.elegan
的细胞蛋白毒性应激反应。
Tags
C. Elegans
Physiological Stress Responses
Stress Response Assay
Heat Shock Protein
Endoplasmic Reticulum Unfolded Protein Response
GFP Reporter
Sodium Azide
Micromanipulation
High-throughput
Genetic Perturbations
Pharmacological Perturbations
Molecular And Physiological Analysis
Synchronization
Imaging
Microscopy
Article
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