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Målinger af fysiologiske stressreaktioner i C. Elegans
JoVE Journal
Biology
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JoVE Journal Biology
Measurements of Physiological Stress Responses in C. Elegans
DOI:

10:36 min

May 21, 2020

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Chapters

  • 00:04Introduction
  • 01:00Stress Response Induction: hsp-4p::GFP Readout
  • 02:11Stereo Microscope or Low-Magnification Wide-Field/Compound Microscope Imaging
  • 04:33Quantitative Reporter Measurement
  • 06:54Stress and Heat Sensitivity Measurement
  • 07:54Results: Representative Cellular Stress Response Evaluation
  • 09:56Conclusion

Summary

Automatic Translation

Her karakteriserer vi cellulære proteotoksiske stressreaktioner i nematoden C. elegans ved at måle aktiveringen af fluorescerende transskriptionelle journalister og assaying følsomhed over for fysiologisk stress.

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