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Hurtig, problemfri generation af rekombinant poxvirus ved hjælp af værtsområde og visuel markering
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Immunology and Infection
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JoVE Journal Immunology and Infection
Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

Hurtig, problemfri generation af rekombinant poxvirus ved hjælp af værtsområde og visuel markering

DOI:
10.3791/61049-v

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09:25 min

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May 24, 2020

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, , , ,
1Department of Medial Microbiology and Immunology,School of Medicine, University of California Davis, 2Department of Microbiology and Immunology,University of Texas Medical Branch at Galveston

Chapters

  • 00:04Introduction
  • 01:03Recombination Vector Generation
  • 03:47Recombinant Virus Generation
  • 06:31Results: Representative Recombinant Poxvirus Generation
  • 08:36Conclusion

Summary

Automatic Translation

Automatic Translation

Dette er en metode til at generere "arløse" rekombinant vaccinia virus ved hjælp af værtsområde udvælgelse og visuel identifikation af rekombinant virus.

Tags

Recombinant PoxvirusesHost Range SelectionFluorescent MarkersHomologous RecombinationTransient Dominant SelectionVaccinia VirusForeign Gene ExpressionVaccinesPCRDNA Gel ExtractionDNA AssemblyE. Coli Transformation

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