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JoVE Journal Cancer Research
Opdagelsen af Driver Gener i Kolorektal HT29-afledt Cancer Stem-Lignende Tumorsfærer
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Opdagelsen af Driver Gener i Kolorektal HT29-afledt Cancer Stem-Lignende Tumorsfærer

Article DOI: 10.3791/61077-v • 06:52 min • July 22nd, 2020
July 22nd, 2020 •
1, 4, 1, 1,2,3
1Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou, 2Department of Medical Imaging and Radiological Sciences, Chang Gung University, 3Department of Radiation Oncology, Chang Gung Memorial Hospital, 4Department of Laboratory Medicine, Mackay Memorial Hospital

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Præsenteret her er en protokol til at opdage de overudtrykte driver gener opretholde de etablerede kræft stamceller-lignende celler stammer fra kolorektal HT29 celler. RNAseq med tilgængelig bioinformatik blev udført for at undersøge og screene genekspressionnetværk for at belyse en potentiel mekanisme, der er involveret i overlevelsen af målrettede tumorceller.

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Tilbagetrækning kolorektal cancer kræft stamceller CD133 HT29 LGR5 RNAseq NetworkAnalyst
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