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Journal
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Immunology and Infection
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在球蛋白细胞炎中进行半细胞外DNA半定量的受监督机器学习
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis
Please note that all translations are automatically generated.
Click here for the English version.
在球蛋白细胞炎中进行半细胞外DNA半定量的受监督机器学习
DOI:
10.3791/61180-v
•
09:16 min
•
June 18, 2020
•
Kim Maree O’Sullivan
,
Sarah Creed
,
Poh-Yi Gan
,
Stephen R. Holdsworth
3
1
Department of Medicine
,
Monash University
,
2
Monash Micro Imaging
,
Monash University
,
3
Immunology Department
,
Monash Health
Chapters
00:04
Introduction
01:00
Extracellular DNA (ecDNA) Staining
03:50
Staining Analysis
07:18
Results: Representative ecDNA Identification and Quantification
08:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
细胞外DNA(ecDNA)在细胞死亡期间释放是消炎,并会导致炎症。在损伤部位测量ecDNA可以确定靶器官治疗的有效性。该协议描述了使用机器学习工具自动测量肾脏组织中 ecDNA。
Tags
Supervised Machine Learning
Semi-quantification
Extracellular DNA
Glomerulonephritis
Kidney Histology
Antigen Retrieval
Immunofluorescence Staining
Sudan Black Quenching
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