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昆虫关键热极限的高通量测定
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High-Throughput Assays of Critical Thermal Limits in Insects
Please note that all translations are automatically generated.
Click here for the English version.
昆虫关键热极限的高通量测定
DOI:
10.3791/61186-v
•
06:58 min
•
June 15, 2020
•
David N. Awde
,
Tatum E. Fowler
,
Fernan Pérez-Gálvez
,
Mark J. Garcia
,
Nicholas M. Teets
1
Department of Entomology
,
University of Kentucky
Chapters
00:04
Introduction
00:40
Critical Thermal Minima (CT
min
) Assay Setup
01:29
Critical Thermal Minima (CT
min
) Assay
03:46
High-Throughput Heat Knockdown (KD) Assay Setup
04:19
High-Throughput Heat Knockdown (KD) Assay
05:45
Results: Representative Lower and Upper Thermal Limits of Select Lines from the
Drosophila
Genetic Reference Panel (DGRP)
06:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
热极限可以预测生物体所容忍的环境,这是面对快速气候变化的宝贵信息。此处描述的高通量协议用于评估昆虫的关键热最小和热敲取时间。两种协议都最大限度地提高了吞吐量,并最大限度地降低测定的成本。
Tags
Thermal Tolerance
Critical Thermal Limits
Insect
Heat Tolerance
Cold Tolerance
Critical Thermal Minimum
Heat Knockdown Time
High-throughput Assay
Semi-automated
Drosophila
Temperature-controlled Fluid Bath
Thermal Couple
Ethanol
Drosophila Funnel Monitor
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