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昆虫关键热极限的高通量测定
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JoVE Journal Biology
High-Throughput Assays of Critical Thermal Limits in Insects
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昆虫关键热极限的高通量测定

DOI:
10.3791/61186-v

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06:58 min

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June 15, 2020

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, , , ,
1Department of Entomology,University of Kentucky

Chapters

  • 00:04Introduction
  • 00:40Critical Thermal Minima (CTmin) Assay Setup
  • 01:29Critical Thermal Minima (CTmin) Assay
  • 03:46High-Throughput Heat Knockdown (KD) Assay Setup
  • 04:19High-Throughput Heat Knockdown (KD) Assay
  • 05:45Results: Representative Lower and Upper Thermal Limits of Select Lines from theDrosophila Genetic Reference Panel (DGRP)
  • 06:16Conclusion

Summary

Automatic Translation

Automatic Translation

热极限可以预测生物体所容忍的环境，这是面对快速气候变化的宝贵信息。此处描述的高通量协议用于评估昆虫的关键热最小和热敲取时间。两种协议都最大限度地提高了吞吐量，并最大限度地降低测定的成本。

Tags

Keywords: Thermal ToleranceCritical Thermal LimitsInsectHeat ToleranceCold ToleranceCritical Thermal MinimumHeat Knockdown TimeHigh-throughput AssaySemi-automatedDrosophilaTemperature-controlled Fluid BathThermal CoupleEthanolDrosophila Funnel Monitor

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