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Parallel høj gennemløb enkelt molekyle kinetisk analyse for site-specifikke DNA spaltning
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Parallel høj gennemløb enkelt molekyle kinetisk analyse for site-specifikke DNA spaltning

Article DOI: 10.3791/61236-v • 06:51 min • May 6th, 2020
May 6th, 2020 •
1, 1, 2
1Department of Biology, Emmanuel College, 2Department of Chemistry and Physics, Emmanuel College

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En meget parallel metode til måling af den stedspecifikke kavalergang af DNA på et enkelt molekyleniveau er beskrevet. Denne protokol demonstrerer teknikken ved hjælp af begrænsningen endonuclease NDEI. Metoden kan nemt ændres til at studere enhver proces, der resulterer i sted-specifikke DNA spaltning.

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Biologi DNA tøjring enkelt molekyle DNA spaltning protein-DNA interaktioner begrænsning endonuclease sted-specifik DNA bindende
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