Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Paralleler Hochdurchsatz-Single Molecule Kinetic…
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
Paralleler Hochdurchsatz-Single Molecule Kinetic Assay für standortspezifische DNA-Spaltung
Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures
Journal (Biology)
Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures
Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
Journal (Biology)
Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
 
Click here for the English version

Paralleler Hochdurchsatz-Single Molecule Kinetic Assay für standortspezifische DNA-Spaltung

Article DOI: 10.3791/61236-v • 06:51 min • May 6th, 2020
May 6th, 2020 •
1, 1, 2
1Department of Biology, Emmanuel College, 2Department of Chemistry and Physics, Emmanuel College

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Beschrieben wird eine hochparallele Methode zur Messung der standortspezifischen DNA-Spaltung auf einzelmolekülebene. Dieses Protokoll demonstriert die Technik mithilfe der Einschränkung endonuklease NdeI. Die Methode kann leicht modifiziert werden, um jeden Prozess zu untersuchen, der zu einer standortspezifischen DNA-Spaltung führt.

Tags

Biologie Ausgabe 159 DNA-Tethering Einzelmolekül DNA-Spaltung Protein-DNA-Wechselwirkungen Restriktionsendonuklease standortspezifische DNA-Bindung
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter