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应用三维单轴机械刺激生物反应器系统诱导肌生衍生干细胞的畸因分化
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Medicine
Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
应用三维单轴机械刺激生物反应器系统诱导肌生衍生干细胞的畸因分化
DOI:
10.3791/61278-v
•
14:04 min
•
August 01, 2020
•
Ziming Chen*
1
,
Peilin Chen*
1
,
Rui Ruan*
1
,
Lianzhi Chen
,
Jun Yuan
,
David Wood
,
Tao Wang
,
Ming Hao Zheng
1
Centre of Orthopaedic Translational Research, Medical School
,
University of Western Australia
Chapters
00:00
Introduction
02:33
Isolation of Mice TDSCs
03:30
Culture and Expansion of Mice TDSCs
05:12
Preparation of Stimulation Culture Medium for Cell Sheet Formation
05:44
Cell Sheet Formation by Culturing in Stimulation Medium
06:21
Preparation of 3D Tendon Stem Cell Construct
07:50
Assembly of the Uniaxial-Stretching Mechanical Stimulation Complex in Unique designed Bioreactor
09:25
Evaluation of the Mechanical Stimulated
in vitro
Tendon-like Tissue
10:10
Representative Results
11:07
Discussion
Summary
Automatic Translation
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Automatic Translation
三维单轴机械刺激生物反应器系统是肌腱衍生干细胞和新肌腱形成具有致源特异性分化的理想生物反应器。
Tags
Tendon-derived Stem Cells
Mechanical Stimulation
Bioreactor System
Tenogenic Differentiation
Tendinopathy
Cell Culture
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