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培养胚胎高级宫颈神经群中的大鼠交感神经元进行形态学和蛋白质学分析
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal Neuroscience
Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic Analysis
DOI:

14:51 min

September 27, 2020

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Chapters

  • 00:00Introduction
  • 00:09Preparation of Media for Dissection and for Culturing Neurons
  • 00:42Preparation of Plates for Culturing Neurons
  • 01:56Dissection Set-up
  • 02:36Embryo Removal and Preparation
  • 04:17Isolation of the SCG from the Embryo
  • 06:52Post-processing of SCG
  • 11:00Maintenance of the Cultured SCG Neurons and Treatments
  • 13:02Representative Results- Immunostaining
  • 14:00Representative Results- Proteomic Analysis
  • 14:29Conclusion

Summary

Automatic Translation

本文描述了胚胎大鼠交感神经元从优越的宫颈神经的分离和培养。它还为免疫细胞化学染色和制备用于质谱分析的神经元提取物提供了详细的方案。

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