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培养胚胎高级宫颈神经群中的大鼠交感神经元进行形态学和蛋白质学分析
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Neuroscience
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Neuroscience
Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic Analysis
Please note that all translations are automatically generated.
Click here for the English version.
培养胚胎高级宫颈神经群中的大鼠交感神经元进行形态学和蛋白质学分析
DOI:
10.3791/61283-v
•
14:51 min
•
September 27, 2020
•
Megan Holt
,
Brandon Adams
,
Vidya Chandrasekaran
1
Department of Biology
,
Saint Mary’s College of California
Chapters
00:00
Introduction
00:09
Preparation of Media for Dissection and for Culturing Neurons
00:42
Preparation of Plates for Culturing Neurons
01:56
Dissection Set-up
02:36
Embryo Removal and Preparation
04:17
Isolation of the SCG from the Embryo
06:52
Post-processing of SCG
11:00
Maintenance of the Cultured SCG Neurons and Treatments
13:02
Representative Results- Immunostaining
14:00
Representative Results- Proteomic Analysis
14:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文描述了胚胎大鼠交感神经元从优越的宫颈神经的分离和培养。它还为免疫细胞化学染色和制备用于质谱分析的神经元提取物提供了详细的方案。
Tags
Rat Sympathetic Neurons
Embryonic Superior Cervical Ganglia
Morphological Analysis
Proteomic Analysis
Cell Culture
Poly-D-lysine
Dissection Medium
Control Medium
Enzymatic Digestion
Centrifugation
Institutional Animal Care And Use Committee
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