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Culturing Rotte sympatiske neuroner fra Embryonic Superior Cervical Ganglia for morfologisk og proteomisk analyse
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Chapters
- 00:00Introduction
- 00:09Preparation of Media for Dissection and for Culturing Neurons
- 00:42Preparation of Plates for Culturing Neurons
- 01:56Dissection Set-up
- 02:36Embryo Removal and Preparation
- 04:17Isolation of the SCG from the Embryo
- 06:52Post-processing of SCG
- 11:00Maintenance of the Cultured SCG Neurons and Treatments
- 13:02Representative Results- Immunostaining
- 14:00Representative Results- Proteomic Analysis
- 14:29Conclusion
Dette papir beskriver isolation og dyrkning af embryonale rotte sympatiske neuroner fra den overlegne livmoderhalskræft ganglier. Det giver også detaljerede protokoller for immunokytokemiske farvning og til forberedelse af neuronale ekstrakter til massespektrometrisk analyse.
