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Cancer Research

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JoVE Journal Cancer Research
Tredimensionel in vitro biomimetisk model af neuroblastom ved hjælp af kollagenbaserede stilladser
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Tredimensionel in vitro biomimetisk model af neuroblastom ved hjælp af kollagenbaserede stilladser

Article DOI: 10.3791/62627-v • 07:48 min • July 9th, 2021
July 9th, 2021 •
*1,2,3, *1,2,3, 4, 3,5,6, 1,2,3,6,7
1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 7Department of Chemistry, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin 2, Ireland, 5Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland, 7National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland
* These authors contributed equally

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Click here for the English version.

Dette papir viser de trin, der kræves for at frø neuroblastomcellelinjer på tidligere beskrevne tredimensionelle kollagenbaserede stilladser, opretholde cellevækst i en forudbestemt tidsramme og hente stilladser til flere cellevækst- og celleadfærdsanalyser og downstream-applikationer, der kan tilpasses til at tilfredsstille en række eksperimentelle mål.

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Tredimensionel In vitro biomimetisk model neuroblastom kollagenbaserede stilladser pædiatrisk kræft tumorvæv ekstracellulær matrix (ECM) sygdomsprogression patientprognose terapeutisk respons neuroblastomcellelinjer nanohydroxyapatit (nHA) glycosaminoglycaner (GAG'er) knogle- og knoglemarv metastatiske steder porøs struktur cellevedhæftning spredning migration celleklynger terapi in vivo-situation stilladsbaseret kultursystem todimensionel (2D) celle Kultur DNA-kvantificering cellelevedygtighed
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