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JoVE Journal Cancer Research
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En automatiseret differentiale nuklear farvning assay til nøjagtig bestemmelse af Mitocan Cytotoxicity

Article DOI: 10.3791/61295-v • 07:58 min • May 12th, 2020
May 12th, 2020 •
*1, *1, 1
1Department of BioSciences, Rice University
* These authors contributed equally

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Protokollen beskriver en hurtig, høj-gennemløb, pålidelig, billig, og upartisk analyse for effektivt at bestemme cellulære levedygtighed. Denne analyse er især nyttig, når cellernes mitokondrier er blevet beskadiget, hvilket forstyrrer andre analyser. Analysen bruger automatiseret optælling af celler farves med to nukleare farvestoffer – Hoechst 33342 og propidium iodid.

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Denne måned i JoVE High-throughput screening mitokondrier-målrettede forbindelser (mitocans) cytotoksicitet assay leukæmi fluorescens mikroskopi automatiseret celletælling
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