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Journal / Biochemistry / Examen espectrofotométrico para posibles…
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Biochemistry
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JoVE Journal Biochemistry
Examen espectrofotométrico para posibles inhibidores de la citosola glutatión S-transferasas
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Examen espectrofotométrico para posibles inhibidores de la citosola glutatión S-transferasas

Article DOI: 10.3791/61347-v • 14:57 min • October 10th, 2020
October 10th, 2020 •
1,2, *1,3, *1,3
1Research platform of Pediatric Onco-Hematology, Department of Paediatrics, Gynaecology and Obstetrics, Faculty of Medicine, University of Geneva, 2Section of Biology, Faculty of Science, University of Geneva, 3Onco-Hematology Unit, Department of Women, Children-Adolescents, University Hospitals of Geneva
* These authors contributed equally

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Glutatión S-transferasas (GST) son enzimas de desintoxicación implicadas en el metabolismo de numerosos fármacos quimioterápticos. La sobreexpresión de los GST está correlacionada con la resistencia a la quimioterapia contra el cáncer. Una manera de contrarrestar este fenotipo es usar inhibidores. Este protocolo describe un método que utiliza un ensayo espectrofotométrico para detectar posibles inhibidores del GST.

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Bioquímica Número 164 Glutatión S-transferasas inhibidores del GST ensayo de enzimas ensayo de inhibición IC50 Ki
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