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Journal / Genetics / Embryo Microinjection Teknikker til effektiv…
JoVE Journal
Genetics

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JoVE Journal Genetics
Embryo Microinjection Teknikker til effektiv Site-Specifikke Myten i Culex quinquefasciatus
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Embryo Microinjection Teknikker til effektiv Site-Specifikke Myten i Culex quinquefasciatus

Article DOI: 10.3791/61375-v • 05:59 min • May 24th, 2020
May 24th, 2020 •
1, 1, 1, 2, 1,3
1Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology and Plant Patholog, Auburn University, 3Tata Institute for Genetics and Society, University of California, San Diego

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Denne protokol beskriver mikroinjection procedurer for Culex quinquefasciatus embryoner, der er optimeret til at arbejde med CRISPR/Cas9 genredigeringsværktøjer. Denne teknik kan effektivt generere stedsspecifikke, arvelige, kønsceller mutationer, der kan bruges til at opbygge genetiske teknologier i denne understudied sygdom vektor.

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Genetik Culex quinquefasciatus CRISPR/Cas9 genomredigering embryonjustering embryomikroinjection genommodifikation kønscellers mytterigenese
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