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JoVE Journal
Genetics

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胚胎微注射技术,用于在库莱克斯五角星高效位点特异性突变体
 
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胚胎微注射技术,用于在库莱克斯五角星高效位点特异性突变体

Article DOI: 10.3791/61375-v
May 24th, 2020

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Summary May 24th, 2020

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Click here for the English version.

该协议描述了 Culex五角星胚胎的 微注射程序,这些胚胎通过CRISPR/Cas9基因编辑工具进行优化。该技术可以有效地生成特定地点、遗传的生殖系突变,可用于在这种研究不足的疾病载体中构建遗传技术。

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