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Cancer Research
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监测三D培养中的癌细胞入侵和T细胞毒性
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture
Please note that all translations are automatically generated.
Click here for the English version.
监测三D培养中的癌细胞入侵和T细胞毒性
DOI:
10.3791/61392-v
•
07:44 min
•
June 23, 2020
•
Yuan-Na Lin
,
Apsra Nasir
,
Sharon Camacho
,
Deborah L. Berry
,
Marcel O. Schmidt
,
Gray W. Pearson
,
Anna T. Riegel
,
Anton Wellstein
1
Lombardi Comprehensive Cancer Center, Department of Oncology
,
Georgetown University
Chapters
00:04
Introduction
00:37
Spheroid Generation
01:43
T Cell Co-Culture
02:39
3D Co-Culture Embedding
03:41
Immunofluorescence StainingImmunofluorescence Staining
05:43
Results: Representative Cancer Cell Invasion Monitoring
07:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方法同时评价了三D球类测定和T细胞毒性中的癌细胞入侵。球体在无脚手架的阿加罗斯多微井铸件中生成。I型胶原蛋白基质中的共同培养和嵌入在同一设备中进行,允许监测癌细胞入侵和T细胞中角细胞毒性。
Tags
3D Culture
Cancer Cell Invasion
T-cell Cytotoxicity
Spheroid Co-culture
Agarose Mold
Tumor Cell Suspension
T-cell Co-culture
Type I Collagen
3D Co-culture
Mechanistic Insights
Immune Cell-mediated Tumor Cell Cytotoxicity
Invasive Behavior
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