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Chapters
- 00:04Introduction
- 00:37Spheroid Generation
- 01:43T Cell Co-Culture
- 02:393D Co-Culture Embedding
- 03:41Immunofluorescence StainingImmunofluorescence Staining
- 05:43Results: Representative Cancer Cell Invasion Monitoring
- 07:18Conclusion
The presented approach simultaneously evaluates cancer cell invasion in 3D spheroid assays and T-cell cytotoxicity. Spheroids are generated in a scaffold-free agarose multi-microwell cast. Co-culture and embedding in type I collagen matrix are performed within the same device which allows to monitor cancer cell invasion and T-cell mediated cytotoxicity.
