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JoVE Journal
Cancer Research

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Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture
 

Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture

Article DOI: 10.3791/61392-v
June 23rd, 2020

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Summary June 23rd, 2020

The presented approach simultaneously evaluates cancer cell invasion in 3D spheroid assays and T-cell cytotoxicity. Spheroids are generated in a scaffold-free agarose multi-microwell cast. Co-culture and embedding in type I collagen matrix are performed within the same device which allows to monitor cancer cell invasion and T-cell mediated cytotoxicity.

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