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Journal / Biochemistry / Het meten van nucleotidebinding aan intacte,…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Het meten van nucleotidebinding aan intacte, functionele membraaneiwitten in realtime
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Het meten van nucleotidebinding aan intacte, functionele membraaneiwitten in realtime

Article DOI: 10.3791/61401-v • 08:33 min • March 11th, 2021
March 11th, 2021 •
1, 1, 1,2
1Department of Physiology, Anatomy and Genetics, University of Oxford, 2Department of Chemistry and Neuroscience Program, Trinity College

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Dit protocol presenteert een methode voor het meten van adenine nucleotide binding aan receptoren in real time in een cellulaire omgeving. Binding wordt gemeten als Förster resonantie energieoverdracht (FRET) tussen trinitrofenylnucleotidederivaten en eiwit gelabeld met een niet-canoniek, fluorescerend aminozuur.

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